› 3itw

X-ray diffraction
2.15Å resolution

Crystal structure of TioX from Micromonospora sp. ML1

Released:
DOI: 10.2210/pdb3itw/pdb
PDB entry 3itw coloured by chain, front view.
PDB entry 3itw coloured by chain, side view.
PDB entry 3itw coloured by chain, top view.
Source organism: Micromonospora sp. ML1
Primary publication:
A new scaffold of an old protein fold ensures binding to the bisintercalator thiocoraline.
Biswas T, Zolova OE, Lombó F, de la Calle F, Salas JA, Tsodikov OV, Garneau-Tsodikova S
J Mol Biol 397 495-507 (2010)
PMID: 20122935

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.47 KDa
Source organism: Micromonospora sp. ML1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q333U2 (Residues: 1-134; Coverage: 100%)
Gene name: tioX
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I4122
Unit cell:
a: 109.321Å b: 109.321Å c: 143.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.268
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Recent developments in bisintercalator natural products.
Zolova et al. (2010)
2 more

1 mention without citation

Resistance to Enediyne Antitumor Antibiotics by Sequestration.
Chang et al. (2018)