› 3iwr

X-ray diffraction
2.57Å resolution

Crystal structure of class I chitinase from Oryza sativa L. japonica

Released:
DOI: 10.2210/pdb3iwr/pdb
PDB entry 3iwr coloured by chain, front view.
PDB entry 3iwr coloured by chain, side view.
PDB entry 3iwr coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structure of full-length class I chitinase from rice revealed by X-ray crystallography and small-angle X-ray scattering.
Kezuka Y, Kojima M, Mizuno R, Suzuki K, Watanabe T, Nonaka T
Proteins 78 2295-305 (2010)
PMID: 20544965

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 32.74 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7DNA1 (Residues: 33-340; Coverage: 100%)
Gene names: Cht2, LOC_Os05g33130, Os05g0399300, OsJ_18467, RC7
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P65
Unit cell:
a: 117.082Å b: 117.082Å c: 77.971Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.236
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural and functional evolution of chitinase-like proteins from plants.
Kesari et al. (2015)
3 more

2 mentions without citation

Chitinase: diversity, limitations, and trends in engineering for suitable applications.
Oyeleye et al. (2018)
1 more