3j45

Electron Microscopy
9.5Å resolution

Structure of a non-translocating SecY protein channel with the 70S ribosome

Released:
Source organism: Escherichia coli
Related structures: EMD-5692

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-141619 (preferred)
Entry contents:
6 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (11 distinct):
Protein translocase subunit SecY Chain: y
Molecule details ›
Chain: y
Length: 437 amino acids
Theoretical weight: 47.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGA2 (Residues: 6-440; Coverage: 98%)
Gene names: JW3262, b3300, prlA, secY
Sequence domains: SecY
Protein translocase subunit SecE Chain: E
Molecule details ›
Chain: E
Length: 56 amino acids
Theoretical weight: 6.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG96 (Residues: 74-127; Coverage: 43%)
Gene names: JW3944, b3981, prlG, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein-export membrane protein SecG Chain: G
Molecule details ›
Chain: G
Length: 65 amino acids
Theoretical weight: 6.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG99 (Residues: 9-73; Coverage: 59%)
Gene names: JW3142, b3175, secG
Sequence domains: Preprotein translocase SecG subunit
Large ribosomal subunit protein uL23 Chain: T
Molecule details ›
Chain: T
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-100; Coverage: 100%)
Gene names: JW3280, b3318, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: U
Molecule details ›
Chain: U
Length: 103 amino acids
Theoretical weight: 11.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60624 (Residues: 2-104; Coverage: 99%)
Gene names: JW3271, b3309, rplX
Sequence domains:
Large ribosomal subunit protein uL29 Chain: Y
Molecule details ›
Chain: Y
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Sequence domains: Ribosomal L29 protein
23S ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 63 nucleotides
Theoretical weight: 20.35 KDa
23S ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 36 nucleotides
Theoretical weight: 11.66 KDa
23S ribosomal RNA Chain: 3
Molecule details ›
Chain: 3
Length: 18 nucleotides
Theoretical weight: 5.8 KDa
23S ribosomal RNA Chain: 4
Molecule details ›
Chain: 4
Length: 61 nucleotides
Theoretical weight: 19.78 KDa
23S ribosomal RNA Chain: 5
Molecule details ›
Chain: 5
Length: 108 nucleotides
Theoretical weight: 34.81 KDa
Sequence domains: Bacterial large subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.5Å
Relevant EMDB volumes: EMD-5692
Expression system: Escherichia coli BL21(DE3)