3j46

Electron Microscopy
10.1Å resolution

Structure of the SecY protein translocation channel in action

Released:
Source organism: Escherichia coli
Related structures: EMD-5693

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-141617 (preferred)
Entry contents:
8 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (14 distinct):
Protein translocase subunit SecY Chain: y
Molecule details ›
Chain: y
Length: 437 amino acids
Theoretical weight: 47.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGA2 (Residues: 6-440; Coverage: 98%)
Gene names: JW3262, b3300, prlA, secY
Sequence domains: SecY
Protein translocase subunit SecE Chain: E
Molecule details ›
Chain: E
Length: 56 amino acids
Theoretical weight: 6.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG96 (Residues: 74-127; Coverage: 43%)
Gene names: JW3944, b3981, prlG, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein-export membrane protein SecG Chain: G
Molecule details ›
Chain: G
Length: 67 amino acids
Theoretical weight: 6.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG99 (Residues: 9-73; Coverage: 59%)
Gene names: JW3142, b3175, secG
Sequence domains: Preprotein translocase SecG subunit
NC100 Chain: n
Molecule details ›
Chain: n
Length: 101 amino acids
Theoretical weight: 10.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL1 Chain: 5
Molecule details ›
Chain: 5
Length: 234 amino acids
Theoretical weight: 24.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L0 (Residues: 1-234; Coverage: 100%)
Gene names: JW3947, b3984, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein uL23 Chain: T
Molecule details ›
Chain: T
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-100; Coverage: 100%)
Gene names: JW3280, b3318, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: U
Molecule details ›
Chain: U
Length: 103 amino acids
Theoretical weight: 11.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60624 (Residues: 2-104; Coverage: 99%)
Gene names: JW3271, b3309, rplX
Sequence domains:
Large ribosomal subunit protein uL29 Chain: Y
Molecule details ›
Chain: Y
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Sequence domains: Ribosomal L29 protein
P-tRNA Chain: p
Molecule details ›
Chain: p
Length: 76 nucleotides
Theoretical weight: 24.48 KDa
Sequence domains: tRNA
A-tRNA Chain: a
Molecule details ›
Chain: a
Length: 76 nucleotides
Theoretical weight: 24.6 KDa
Sequence domains: tRNA
23S ribosomal RNA Chain: 1
Molecule details ›
Chain: 1
Length: 63 nucleotides
Theoretical weight: 20.35 KDa
23S ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 36 nucleotides
Theoretical weight: 11.66 KDa
23S ribosomal RNA Chain: 3
Molecule details ›
Chain: 3
Length: 44 nucleotides
Theoretical weight: 14.26 KDa
23S ribosomal RNA Chain: 4
Molecule details ›
Chain: 4
Length: 109 nucleotides
Theoretical weight: 35.12 KDa
Sequence domains: Bacterial large subunit ribosomal RNA

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 10.1Å
Relevant EMDB volumes: EMD-5693
Expression system: Escherichia coli