› 3j4s

Electron Microscopy
6.8Å resolution

Helical Model of TubZ-Bt four-stranded filament

Released:
DOI: 10.2210/pdb3j4s/pdb
PDB entry 3j4s coloured by chain, front view.
PDB entry 3j4s coloured by chain, side view.
PDB entry 3j4s coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar israelensis
Primary publication:
Bacterial tubulin TubZ-Bt transitions between a two-stranded intermediate and a four-stranded filament upon GTP hydrolysis.
Montabana EA, Agard DA
Proc Natl Acad Sci U S A 111 3407-12 (2014)
PMID: 24550513
Related structures: EMD-5762

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tubulin-like protein TubZ Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 54.44 KDa
Source organism: Bacillus thuringiensis serovar israelensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KNP3 (Residues: 1-484; Coverage: 100%)
Gene names: ATN07_33550, pBt156, tubZ

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.8Å
Relevant EMDB volumes: EMD-5762
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Prokaryotic cytoskeletons: protein filaments organizing small cells.
Wagstaff et al. (2018)
5 more

1 mention without citation

Bacterial tubulin TubZ-Bt transitions between a two-stranded intermediate and a four-stranded filament upon GTP hydrolysis.
Montabana et al. (2014)