› 3j6f

Electron Microscopy
4.9Å resolution

Minimized average structure of GDP-bound dynamic microtubules

Released:
DOI: 10.2210/pdb3j6f/pdb
PDB entry 3j6f coloured by chain, front view.
PDB entry 3j6f coloured by chain, side view.
PDB entry 3j6f coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
High-resolution microtubule structures reveal the structural transitions in αβ-tubulin upon GTP hydrolysis.
Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E
Cell 157 1117-29 (2014)
PMID: 24855948
Related structures: EMD-5896

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135909 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin alpha-1A chain Chains: A, C, E, G, I, K, M, O, Q
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q
Length: 439 amino acids
Theoretical weight: 48.77 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02550 (Residues: 1-439; Coverage: 97%)
Gene name: TUBA1A
Sequence domains:
Tubulin beta chain Chains: B, D, F, H, J, L, N, P, R
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R
Length: 427 amino acids
Theoretical weight: 47.94 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-427; Coverage: 96%)
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 9 x GTP
Ligand MG 9 x MG
Ligand GDP 9 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.9Å
Relevant EMDB volumes: EMD-5896

Quick links

Citations

68 review citations

Control of microtubule organization and dynamics: two ends in the limelight.
Akhmanova et al. (2015)
67 more

14 mentions without citation

Modeling the Dynamics of Protein-Protein Interfaces, How and Why?
Karaca et al. (2022)
13 more