› 3j94

Electron Microscopy
4.2Å resolution

Structure of ATP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopy

Released:
DOI: 10.2210/pdb3j94/pdb
PDB entry 3j94 coloured by chain, front view.
PDB entry 3j94 coloured by chain, side view.
PDB entry 3j94 coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Mechanistic insights into the recycling machine of the SNARE complex.
Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT
Nature 518 61-7 (2015)
PMID: 25581794
Related structures: EMD-6204

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vesicle-fusing ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 747 amino acids
Theoretical weight: 82.91 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18708 (Residues: 1-744; Coverage: 100%)
Gene name: NSF
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 11 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-6204
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines.
Olivares et al. (2016)
40 more

14 mentions without citation

Chaperoning SNARE assembly and disassembly.
Baker et al. (2016)
13 more