› 3jtj

X-ray diffraction
2.18Å resolution

3-deoxy-manno-octulosonate cytidylyltransferase from Yersinia pestis

Released:
DOI: 10.2210/pdb3jtj/pdb
PDB entry 3jtj coloured by chain, front view.
PDB entry 3jtj coloured by chain, side view.
PDB entry 3jtj coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Osipiuk J, Zhou M, Grimshaw S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-deoxy-manno-octulosonate cytidylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.92 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZGA4 (Residues: 1-250; Coverage: 100%)
Gene names: YPO1400, YP_1193, kdsB, y2772
Sequence domains: Cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

1 bound ligand:
Ligand IMD 2 x IMD
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 97.058Å b: 97.058Å c: 93.626Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.209
Expression system: Escherichia coli

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