› 3ju8

X-ray diffraction
1.82Å resolution

Crystal Structure of Succinylglutamic Semialdehyde Dehydrogenase from Pseudomonas aeruginosa.

Released:
DOI: 10.2210/pdb3ju8/pdb
PDB entry 3ju8 coloured by chain, front view.
PDB entry 3ju8 coloured by chain, side view.
PDB entry 3ju8 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Kim Y, Li H, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-succinylglutamate 5-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 52 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O50174 (Residues: 1-486; Coverage: 100%)
Gene names: PA0898, aruD, astD
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
5 bound ligands:
Ligand SO4 8 x SO4
Ligand SIN 1 x SIN
Ligand GOL 8 x GOL
Ligand CL 2 x CL
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 137.117Å b: 48.033Å c: 187.13Å
α: 90° β: 107.43° γ: 90°
R-values:
R R work R free
0.16 0.158 0.196
Expression system: Escherichia coli BL21

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