› 3juy

X-ray diffraction
2.5Å resolution

Crystal Structure of a 3B3 Variant, a Broadly Neutralizing HIV-1 scFv Antibody

Released:
DOI: 10.2210/pdb3juy/pdb
PDB entry 3juy coloured by chain, front view.
PDB entry 3juy coloured by chain, side view.
PDB entry 3juy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a 3B3 variant--a broadly neutralizing HIV-1 scFv antibody.
Clark KR, Walsh ST
Protein Sci 18 2429-41 (2009)
PMID: 19785005

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3B3 single chain variant HIV-1 antibody Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 256 amino acids
Theoretical weight: 28.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 199.172Å b: 115.13Å c: 92.374Å
α: 90° β: 111.76° γ: 90°
R-values:
R R work R free
0.201 0.195 0.26
Expression system: Escherichia coli BL21

Quick links

Citations

8 citation in other articles

Specific fluorine labeling of the HyHEL10 antibody affects antigen binding and dynamics.
Acchione et al. (2012)
7 more

8 mentions without citation

Crystal structure of a 3B3 variant--a broadly neutralizing HIV-1 scFv antibody.
Clark et al. (2009)
7 more