› 3jvt

X-ray diffraction
2.1Å resolution

Calcium-bound Scallop Myosin Regulatory Domain (Lever Arm) with Reconstituted Complete Light Chains

Released:
DOI: 10.2210/pdb3jvt/pdb
PDB entry 3jvt coloured by chain, front view.
PDB entry 3jvt coloured by chain, side view.
PDB entry 3jvt coloured by chain, top view.
Source organism: Argopecten irradians
Primary publication:
The on-off switch in regulated myosins: different triggers but related mechanisms.
Himmel DM, Mui S, O'Neall-Hennessey E, Szent-Györgyi AG, Cohen C
J Mol Biol 394 496-505 (2009)
PMID: 19769984

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139482 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin heavy chain, striated muscle Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 8.13 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P24733 (Residues: 773-837; Coverage: 3%)
Structure domains: Myosin, subunit A
Myosin regulatory light chain, striated adductor muscle Chain: B
Molecule details ›
Chain: B
Length: 156 amino acids
Theoretical weight: 17.56 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P13543 (Residues: 2-157; Coverage: 99%)
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Myosin essential light chain, striated adductor muscle Chain: C
Molecule details ›
Chain: C
Length: 156 amino acids
Theoretical weight: 17.64 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P07291 (Residues: 2-157; Coverage: 99%)
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P1
Unit cell:
a: 46.71Å b: 52.23Å c: 55.25Å
α: 65.3° β: 66.4° γ: 70.24°
R-values:
R R work R free
0.209 0.207 0.231

Quick links

Citations

3 review citations

Myosin light chains: Teaching old dogs new tricks.
Heissler et al. (2014)
2 more

3 mentions without citation

Structural and energetic determinants of apo calmodulin binding to the IQ motif of the Na(V)1.2 voltage-dependent sodium channel.
Feldkamp et al. (2011)
2 more