› 3k0b

X-ray diffraction
1.5Å resolution

Crystal structure of a predicted N6-adenine-specific DNA methylase from Listeria monocytogenes str. 4b F2365

Released:
DOI: 10.2210/pdb3k0b/pdb
PDB entry 3k0b coloured by chain, front view.
PDB entry 3k0b coloured by chain, side view.
PDB entry 3k0b coloured by chain, top view.
Source organism: Listeria monocytogenes serotype 4b str. F2365
Entry authors: Nocek B, Xu X, Cui H, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
predicted N6-adenine-specific DNA methylase Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 44.61 KDa
Source organism: Listeria monocytogenes serotype 4b str. F2365
Expression system: Escherichia coli BL21
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 125.949Å b: 46.156Å c: 91.376Å
α: 90° β: 131.09° γ: 90°
R-values:
R R work R free
0.17 0.164 0.198
Expression system: Escherichia coli BL21

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