› 3k0t

X-ray diffraction
2.1Å resolution

Crystal structure of PSPTO -PSP protein in complex with D-beta-Glucose from Pseudomonas syringae pv. tomato str. DC3000

Released:
DOI: 10.2210/pdb3k0t/pdb
PDB entry 3k0t coloured by chain, front view.
PDB entry 3k0t coloured by chain, side view.
PDB entry 3k0t coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato
Primary publication:
Crystal structure of the PSPTO-PSP protein from Pseudomonas syringae pv. tomato str. DC3000 in complex with D-glucose.
Zhang HM, Gao Y, Li M, Chang WR
Biochem Biophys Res Commun 397 82-6 (2010)
PMID: 20478270

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease L-PSP, putative Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 143 amino acids
Theoretical weight: 15.7 KDa
Source organism: Pseudomonas syringae pv. tomato
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88BE5 (Residues: 3-126; Coverage: 98%)
Gene name: PSPTO_0072
Sequence domains: Endoribonuclease L-PSP
Structure domains: RutC-like

Ligands and Environments

1 bound ligand:
Ligand BGC 3 x BGC
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 66.896Å b: 75.714Å c: 79.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.237
Expression system: Escherichia coli

Quick links

Citations

2 review citations

From microbiology to cancer biology: the Rid protein family prevents cellular damage caused by endogenously generated reactive nitrogen species.
Downs et al. (2015)
1 more

3 mentions without citation

RidA Proteins Protect against Metabolic Damage by Reactive Intermediates.
Irons et al. (2020)
2 more