› 3k1y

X-ray diffraction
2.5Å resolution

X-ray structure of oxidoreductase from corynebacterium diphtheriae. orthorombic crystal form, northeast structural genomics consortium target cdr100d

Released:
DOI: 10.2210/pdb3k1y/pdb
PDB entry 3k1y coloured by chain, front view.
PDB entry 3k1y coloured by chain, side view.
PDB entry 3k1y coloured by chain, top view.
Source organism: Corynebacterium diphtheriae
Entry authors: Kuzin A, Lew S, Sahdev S, Xiao R, Ciccosanti C, Wang H, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent FMN reductase-like domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 191 amino acids
Theoretical weight: 20.46 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6NGS1 (Residues: 1-181; Coverage: 82%)
Gene name: DIP1437
Sequence domains: NADPH-dependent FMN reductase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 16 x SO4
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 87.17Å b: 141.379Å c: 201.565Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.241
Expression system: Escherichia coli BL21(DE3)

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