› 3k28

X-ray diffraction
1.95Å resolution

Crystal Structure of a glutamate-1-semialdehyde aminotransferase from Bacillus anthracis with bound Pyridoxal 5'Phosphate

Released:
DOI: 10.2210/pdb3k28/pdb
PDB entry 3k28 coloured by chain, front view.
PDB entry 3k28 coloured by chain, side view.
PDB entry 3k28 coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Sharma SS, Brunzelle JS, Wawrzak Z, Skarina T, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde 2,1-aminomutase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 429 amino acids
Theoretical weight: 46.76 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81LD0 (Residues: 1-429; Coverage: 100%)
Gene names: BAS4358, BA_4693, GBAA_4693, hemL2
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
Ligand CA 10 x CA
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 149.809Å b: 113.688Å c: 113.955Å
α: 90° β: 118.65° γ: 90°
R-values:
R R work R free
0.147 0.147 0.185
Expression system: Escherichia coli BL21(DE3)

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