› 3k2n

X-ray diffraction
2.5Å resolution

The crystal structure of sigma-54-dependent transcriptional regulator domain from Chlorobium Tepidum TLS

Released:
DOI: 10.2210/pdb3k2n/pdb
PDB entry 3k2n coloured by chain, front view.
PDB entry 3k2n coloured by chain, side view.
PDB entry 3k2n coloured by chain, top view.
Source organism: Chlorobaculum tepidum TLS
Entry authors: Wu R, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sigma-54 factor interaction domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.91 KDa
Source organism: Chlorobaculum tepidum TLS
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8KG60 (Residues: 30-203; Coverage: 24%)
Gene name: CT0108
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 55.121Å b: 81.25Å c: 83.522Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.197 0.28
Expression system: Escherichia coli BL21

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