› 3k4h

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF putative transcriptional regulator LacI from Bacillus cereus subsp. cytotoxis NVH 391-98

Released:
DOI: 10.2210/pdb3k4h/pdb
PDB entry 3k4h coloured by chain, front view.
PDB entry 3k4h coloured by chain, side view.
PDB entry 3k4h coloured by chain, top view.
Source organism: Bacillus cytotoxicus NVH 391-98
Entry authors: Malashkevich VN, Toro R, Morano C, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Transcriptional regulator LacI/GalR-like sensor domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 32.64 KDa
Source organism: Bacillus cytotoxicus NVH 391-98
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0VWZ8 (Residues: 1-292; Coverage: 100%)
Gene names: Bcer98_2711, Q2E7G6
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 98.607Å b: 98.607Å c: 186.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.244
Expression system: Escherichia coli BL21(DE3)

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