› 3k4i

X-ray diffraction
1.69Å resolution

CRYSTAL STRUCTURE OF uncharacterized protein PSPTO_3204 from Pseudomonas syringae pv. tomato str. DC3000

Released:
DOI: 10.2210/pdb3k4i/pdb
PDB entry 3k4i coloured by chain, front view.
PDB entry 3k4i coloured by chain, side view.
PDB entry 3k4i coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato
Entry authors: Malashkevich VN, Toro R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reactions catalysed: 
Oxaloacetate = pyruvate + CO(2)
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = oxaloacetate + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-4-methyl-2-oxoglutarate aldolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 244 amino acids
Theoretical weight: 26.54 KDa
Source organism: Pseudomonas syringae pv. tomato
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q880F6 (Residues: 2-234; Coverage: 98%)
Gene name: PSPTO_3204
Sequence domains: Aldolase/RraA
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand MG 3 x MG
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 34.442Å b: 124.857Å c: 66.912Å
α: 90° β: 97.99° γ: 90°
R-values:
R R work R free
0.18 0.178 0.214
Expression system: Escherichia coli BL21(DE3)

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