› 3k4u

X-ray diffraction
2.62Å resolution

CRYSTAL STRUCTURE OF putative binding component of ABC transporter from Wolinella succinogenes DSM 1740 complexed with lysine

Released:
DOI: 10.2210/pdb3k4u/pdb
PDB entry 3k4u coloured by chain, front view.
PDB entry 3k4u coloured by chain, side view.
PDB entry 3k4u coloured by chain, top view.
Source organism: Wolinella succinogenes
Entry authors: Malashkevich VN, Toro R, Morano C, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo pentamer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-181712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BINDING COMPONENT OF ABC TRANSPORTER Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 245 amino acids
Theoretical weight: 28.34 KDa
Source organism: Wolinella succinogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MAG0 (Residues: 34-267; Coverage: 88%)
Gene name: WS0279
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand LYS 6 x LYS
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 79.406Å b: 149.151Å c: 81.969Å
α: 90° β: 100.46° γ: 90°
R-values:
R R work R free
0.205 0.202 0.266
Expression system: Escherichia coli BL21(DE3)

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