› 3k85

X-ray diffraction
2.28Å resolution

Crystal structure of a D-glycero-D-manno-heptose 1-phosphate kinase from Bacteriodes thetaiotaomicron

Released:
DOI: 10.2210/pdb3k85/pdb
PDB entry 3k85 coloured by chain, front view.
PDB entry 3k85 coloured by chain, side view.
PDB entry 3k85 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Entry authors: Palani K, Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-183788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-glycero-D-manno-heptose 1-phosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 357 amino acids
Theoretical weight: 39.79 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8AAJ0 (Residues: 3-348; Coverage: 99%)
Gene name: BT_0474
Sequence domains:
Structure domains: Ribosomal Protein S5; domain 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 71.889Å b: 149.469Å c: 141.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.282
Expression system: Escherichia coli BL21(DE3)

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