› 3k8o

X-ray diffraction
2.4Å resolution

Crystal structure of human purine nucleoside phosphorylase in complex with DATMe-ImmH

Released:
DOI: 10.2210/pdb3k8o/pdb
PDB entry 3k8o coloured by chain, front view.
PDB entry 3k8o coloured by chain, side view.
PDB entry 3k8o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human purine nucleoside phosphorylase in complex with DATMe-Immucillin H
Ho M, Rinaldo-matthis A, Almo SC, Schramm VL
Proc. Natl. Acad. Sci. U.S.A. (2010)

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chains: E, Q, S, T, U, Y
Molecule details ›
Chains: E, Q, S, T, U, Y
Length: 289 amino acids
Theoretical weight: 32.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00491 (Residues: 1-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand SO4 16 x SO4
Ligand 229 6 x 229
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 269.212Å b: 51.875Å c: 129.069Å
α: 90° β: 90.44° γ: 90°
R-values:
R R work R free
0.226 0.223 0.272
Expression system: Escherichia coli BL21(DE3)

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