› 3k9w

X-ray diffraction
1.6Å resolution

Crystal structure of phosphopantetheine adenylyltransferase from Burkholderia pseudomallei with hydrolyzed 3'-dephospho Coenzyme A

Released:
DOI: 10.2210/pdb3k9w/pdb
PDB entry 3k9w coloured by chain, front view.
PDB entry 3k9w coloured by chain, side view.
PDB entry 3k9w coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Primary publication:
Structures of phosphopantetheine adenylyltransferase from Burkholderia pseudomallei.
Edwards TE, Leibly DJ, Bhandari J, Statnekov JB, Phan I, Dieterich SH, Abendroth J, Staker BL, Van Voorhis WC, Myler PJ, Stewart LJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1032-7 (2011)
PMID: 21904046

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 20.84 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JW91 (Residues: 1-166; Coverage: 100%)
Gene names: BURPS1710b_0748, coaD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

5 bound ligands:
Ligand 4PS 1 x 4PS
Ligand ADE 1 x ADE
Ligand PG4 1 x PG4
Ligand ACT 1 x ACT
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I432
Unit cell:
a: 134.431Å b: 134.431Å c: 134.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural Biology and the Design of New Therapeutics: From HIV and Cancer to Mycobacterial Infections: A Paper Dedicated to John Kendrew.
Thomas et al. (2017)
1 other article

3 mentions without citation

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh et al. (2013)
2 more