› 3ka5

X-ray diffraction
1.8Å resolution

Crystal structure of Ribosome-associated protein Y (PSrp-1) from Clostridium acetobutylicum. Northeast Structural Genomics Consortium target id CaR123A

Released:
DOI: 10.2210/pdb3ka5/pdb
PDB entry 3ka5 coloured by chain, front view.
PDB entry 3ka5 coloured by chain, side view.
PDB entry 3ka5 coloured by chain, top view.
Source organism: Clostridium acetobutylicum
Entry authors: Seetharaman J, Neely H, Wang D, Janjua H, Cunningham K, Owens L, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome hibernation promoting factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 7.78 KDa
Source organism: Clostridium acetobutylicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97F93 (Residues: 117-173; Coverage: 33%)
Gene names: CA_C2847, hpf
Sequence domains: Sigma 54 modulation/S30EA ribosomal protein C terminus

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P3121
Unit cell:
a: 49.693Å b: 49.693Å c: 98.382Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.233
Expression system: Escherichia coli

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