› 3kao

X-ray diffraction
1.9Å resolution

Crystal structure of tagatose 1,6-diphosphate aldolase from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3kao/pdb
PDB entry 3kao coloured by chain, front view.
PDB entry 3kao coloured by chain, side view.
PDB entry 3kao coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Entry authors: Chang C, Marshall N, Cobb G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tagatose 1,6-diphosphate aldolase Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 37.15 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A009 (Residues: 1-326; Coverage: 100%)
Gene names: SAV2192, lacD
Sequence domains: DeoC/LacD family aldolase
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ZN 3 x ZN
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 84.704Å b: 84.704Å c: 202.759Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.171
Expression system: Escherichia coli BL21(DE3)

Quick links