› 3kcc

X-ray diffraction
1.66Å resolution

Crystal structure of D138L mutant of Catabolite Gene Activator Protein

Released:
DOI: 10.2210/pdb3kcc/pdb
PDB entry 3kcc coloured by chain, front view.
PDB entry 3kcc coloured by chain, side view.
PDB entry 3kcc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The 1.6Å resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer.
Tao W, Gao Z, Gao Z, Zhou J, Huang Z, Dong Y, Yu S
Int J Biol Macromol 48 459-65 (2011)
PMID: 21255606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional dual regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 29.11 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CMP 4 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 45.536Å b: 102.222Å c: 53.912Å
α: 90° β: 110.93° γ: 90°
R-values:
R R work R free
0.206 0.204 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Neuromodulators as Interdomain Signaling Molecules Capable of Occupying Effector Binding Sites in Bacterial Transcription Factors.
Purtov et al. (2023)

1 mention without citation

Dynamic Allostery of the Catabolite Activator Protein Revealed by Interatomic Forces.
Louet et al. (2015)