› 3ke0

X-ray diffraction
2.7Å resolution

Crystal structure of N370S Glucocerebrosidase at acidic pH.

Released:
DOI: 10.2210/pdb3ke0/pdb
PDB entry 3ke0 coloured by chain, front view.
PDB entry 3ke0 coloured by chain, side view.
PDB entry 3ke0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wei RR, Boucher S, Pan CQ, Edmunds T

Function and Biology Details

Reactions catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-137378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Lysosomal acid glucosylceramidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 55.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand SO4 13 x SO4
Ligand GOL 3 x GOL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2221
Unit cell:
a: 109.465Å b: 285.064Å c: 92.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.229
Expression system: Spodoptera frugiperda

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