› 3ket

X-ray diffraction
2.4Å resolution

Crystal structure of a Rex-family transcriptional regulatory protein from Streptococcus agalactiae bound to a palindromic operator

Released:
DOI: 10.2210/pdb3ket/pdb
PDB entry 3ket coloured by chain, front view.
PDB entry 3ket coloured by chain, side view.
PDB entry 3ket coloured by chain, top view.
Source organisms:
Primary publication:
NAD+ pool depletion as a signal for the Rex regulon involved in Streptococcus agalactiae virulence.
Franza T, Rogstam A, Thiyagarajan S, Sullivan MJ, Derré-Bobillot A, Bauer MC, Goh KGK, Da Cunha V, Glaser P, Logan DT, Ulett GC, von Wachenfeldt C, Gaudu P
PLoS Pathog 17 e1009791 (2021)
PMID: 34370789

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116476 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Redox-sensing transcriptional repressor Rex Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.85 KDa
Source organism: Streptococcus agalactiae serogroup III
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8E565 (Residues: 1-212; Coverage: 100%)
Gene names: gbs1167, rex
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*TP*TP*GP*TP*GP*AP*AP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.4 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*TP*TP*TP*CP*AP*CP*AP*AP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.31 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NAD 1 x NAD
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: I222
Unit cell:
a: 48.72Å b: 107.03Å c: 153.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Butyrate enhances Clostridioides difficile sporulation in vitro.
Baldassare et al. (2023)

4 mentions without citation

Transcriptional regulation of central carbon and energy metabolism in bacteria by redox-responsive repressor Rex.
Ravcheev et al. (2012)
3 more