› 3kev

X-ray diffraction
1.3Å resolution

X-ray crystal structure of a DCUN1 domain-containing protein from Galdieria sulfuraria

Released:
DOI: 10.2210/pdb3kev/pdb
PDB entry 3kev coloured by chain, front view.
PDB entry 3kev coloured by chain, side view.
PDB entry 3kev coloured by chain, top view.
Source organism: Galdieria sulphuraria
Primary publication:
Structural architecture of Galdieria sulphuraria DCN1L.
Sethe Burgie E, Bingman CA, Makino S, Wesenberg GE, Pan X, Fox BG, Phillips GN
Proteins 79 1329-36 (2011)
PMID: 21387409

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Defective in cullin neddylation protein Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 23.33 KDa
Source organism: Galdieria sulphuraria
Expression system: CELL-FREE SYNTHESIS
UniProt:
  • Canonical: D1MPT4 (Residues: 1-199; Coverage: 100%)
Sequence domains: Cullin binding
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand ACT 2 x ACT
2 modified residues:
Ligand CSO 1 x CSO
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D, APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 36.743Å b: 52.422Å c: 49.113Å
α: 90° β: 111.17° γ: 90°
R-values:
R R work R free
0.156 0.155 0.178
Expression system: CELL-FREE SYNTHESIS

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Citations

1 review citation

Targeting cullin neddylation for cancer and fibrotic diseases.
He et al. (2023)
1 other article

2 mentions without citation

Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins.
Beebe et al. (2011)
1 more