› 3kfd

X-ray diffraction
3Å resolution

Ternary complex of TGF-b1 reveals isoform-specific ligand recognition and receptor recruitment in the superfamily

Released:
DOI: 10.2210/pdb3kfd/pdb
PDB entry 3kfd coloured by chain, front view.
PDB entry 3kfd coloured by chain, side view.
PDB entry 3kfd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ternary complex of transforming growth factor-beta1 reveals isoform-specific ligand recognition and receptor recruitment in the superfamily.
Radaev S, Zou Z, Huang T, Lafer EM, Hinck AP, Sun PD
J Biol Chem 285 14806-14 (2010)
PMID: 20207738

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-134062 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transforming growth factor beta-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 112 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01137 (Residues: 279-390; Coverage: 31%)
Gene names: TGFB, TGFB1
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines
TGF-beta receptor type-2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 116 amino acids
Theoretical weight: 13.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37173 (Residues: 38-153; Coverage: 21%)
Gene name: TGFBR2
Sequence domains: Transforming growth factor beta receptor 2 ectodomain
Structure domains: CD59
TGF-beta receptor type-1 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 85 amino acids
Theoretical weight: 9.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36897 (Residues: 31-115; Coverage: 17%)
Gene names: ALK5, SKR4, TGFBR1
Sequence domains: Activin types I and II receptor domain
Structure domains: CD59

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 37.7Å b: 99.35Å c: 102.7Å
α: 64.01° β: 84.47° γ: 84.34°
R-values:
R R work R free
0.22 0.217 0.268
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

19 review citations

Signaling Receptors for TGF-β Family Members.
Heldin et al. (2016)
18 more

39 mentions without citation

Structural biology of betaglycan and endoglin, membrane-bound co-receptors of the TGF-beta family.
Kim et al. (2019)
38 more