› 3kfo

X-ray diffraction
1.9Å resolution

Crystal structure of the C-terminal domain from the nuclear pore complex component NUP133 from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3kfo/pdb
PDB entry 3kfo coloured by chain, front view.
PDB entry 3kfo coloured by chain, side view.
PDB entry 3kfo coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the C-terminal domain of Saccharomyces cerevisiae Nup133, a component of the nuclear pore complex.
Sampathkumar P, Gheyi T, Miller SA, Bain KT, Dickey M, Bonanno JB, Kim SJ, Phillips J, Pieper U, Fernandez-Martinez J, Franke JD, Martel A, Tsuruta H, Atwell S, Thompson DA, Emtage JS, Wasserman SR, Rout MP, Sali A, Sauder JM, Burley SK
Proteins 79 1672-7 (2011)
PMID: 21365675

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoporin NUP133 Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 33.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36161 (Residues: 881-1157; Coverage: 24%)
Gene names: NUP133, RAT3, YKR082W, YKR402
Sequence domains: Non-repetitive/WGA-negative nucleoporin C-terminal

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 47.433Å b: 52.678Å c: 76.674Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Evolution: On a bender--BARs, ESCRTs, COPs, and finally getting your coat.
Field et al. (2011)
3 more

6 mentions without citation

Integrative structural modeling with small angle X-ray scattering profiles.
Schneidman-Duhovny et al. (2012)
5 more