› 3khe

X-ray diffraction
1.95Å resolution

Crystal structure of the calcium-loaded calmodulin-like domain of the CDPK, 541.m00134 from toxoplasma gondii

Released:
DOI: 10.2210/pdb3khe/pdb
PDB entry 3khe coloured by chain, front view.
PDB entry 3khe coloured by chain, side view.
PDB entry 3khe coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Structures of parasitic CDPK domains point to a common mechanism of activation.
Wernimont AK, Amani M, Qiu W, Pizarro JC, Artz JD, Lin YH, Lew J, Hutchinson A, Hui R
Proteins 79 803-20 (2011)
PMID: 21287613

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-dependent protein kinase CDPK3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.84 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q3HNM6 (Residues: 345-535; Coverage: 36%)
Gene name: TGRH88_017420
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

3 bound ligands:
Ligand CA 8 x CA
Ligand MG 3 x MG
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 45.838Å b: 101.015Å c: 107.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.277
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Calcium-dependent protein kinases: hubs in plant stress signaling and development.
Schulz et al. (2013)
12 more