› 3kio

X-ray diffraction
2.9Å resolution

mouse RNase H2 complex

Released:
DOI: 10.2210/pdb3kio/pdb
PDB entry 3kio coloured by chain, front view.
PDB entry 3kio coloured by chain, side view.
PDB entry 3kio coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction.
Shaban NM, Harvey S, Perrino FW, Hollis T
J Biol Chem 285 3617-3624 (2010)
PMID: 19923215

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182412 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ribonuclease H2 subunit A Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 33.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CWY8 (Residues: 1-301; Coverage: 100%)
Gene names: Rnaseh2a, Rnasehi
Sequence domains: Ribonuclease HII
Structure domains:
Ribonuclease H2 subunit B Chain: B
Molecule details ›
Chain: B
Length: 332 amino acids
Theoretical weight: 37.77 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80ZV0 (Residues: 1-308; Coverage: 100%)
Gene names: Dleu8, Rnaseh2b
Sequence domains:
Ribonuclease H2 subunit C Chain: C
Molecule details ›
Chain: C
Length: 166 amino acids
Theoretical weight: 17.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CQ18 (Residues: 1-166; Coverage: 100%)
Gene names: Ayp1, Rnaseh2c
Sequence domains: Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Structure domains: Lipocalin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 279.287Å b: 40.421Å c: 67.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.234 0.306
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Yin and Yang of R-loop biology.
Costantino et al. (2015)
5 more

6 mentions without citation

The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease.
Reijns et al. (2011)
5 more