› 3kj6

X-ray diffraction
3.4Å resolution

Crystal structure of a Methylated beta2 Adrenergic Receptor-Fab complex

Released:
DOI: 10.2210/pdb3kj6/pdb
PDB entry 3kj6 coloured by chain, front view.
PDB entry 3kj6 coloured by chain, side view.
PDB entry 3kj6 coloured by chain, top view.
Source organisms:
Primary publication:
Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor.
Bokoch MP, Zou Y, Rasmussen SG, Liu CW, Nygaard R, Rosenbaum DM, Fung JJ, Choi HJ, Thian FS, Kobilka TS, Puglisi JD, Weis WI, Pardo L, Prosser RS, Mueller L, Kobilka BK
Nature 463 108-12 (2010)
PMID: 20054398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209317 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-2 adrenergic receptor Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 41.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 2-365; Coverage: 88%)
Gene names: ADRB2, ADRB2R, B2AR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Structure domains: Rhodopsin 7-helix transmembrane proteins
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.9 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.24 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 338.6Å b: 48.6Å c: 88.9Å
α: 90° β: 104.4° γ: 90°
R-values:
R R work R free
0.242 0.238 0.28
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus

Quick links

Citations

84 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
83 more

35 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
34 more