› 3kkb

X-ray diffraction
1.88Å resolution

The crystal structure of a two-component sensor domain from Pseudomonas aeruginosa PA01

Released:
DOI: 10.2210/pdb3kkb/pdb
PDB entry 3kkb coloured by chain, front view.
PDB entry 3kkb coloured by chain, side view.
PDB entry 3kkb coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Tan K, Chhor G, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate biosynthesis sensor protein KinB Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.56 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O34206 (Residues: 42-168; Coverage: 21%)
Gene name: kinB
Sequence domains: Sensor domain of alginate biosynthesis sensor protein KinB
Structure domains: Histidine kinase (KinB), sensor domain

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 75.361Å b: 75.361Å c: 75.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.163 0.22
Expression system: Escherichia coli BL21

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