› 3kkj

X-ray diffraction
2.5Å resolution

X-ray structure of P. syringae q888a4 Oxidoreductase at resolution 2.5A, Northeast Structural Genomics Consortium target PsR10

Released:
DOI: 10.2210/pdb3kkj/pdb
PDB entry 3kkj coloured by chain, front view.
PDB entry 3kkj coloured by chain, side view.
PDB entry 3kkj coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato
Entry authors: Kuzin AP, Chen Y, Forouhar F, Vorobiev S, Acton T, Ma LC, Xiao R, Montelione GT, Hunt JF, Tong T, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
1,2-dihydro-beta-NAD(P) + H(+) + O(2) = beta-NAD(P)(+) + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Renalase Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 37.37 KDa
Source organism: Pseudomonas syringae pv. tomato
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q888A4 (Residues: 1-328; Coverage: 100%)
Gene name: PSPTO_1126
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3221
Unit cell:
a: 120.698Å b: 120.698Å c: 114.791Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.227 0.281
Expression system: Escherichia coli BL21(DE3)

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