› 3kkz

X-ray diffraction
1.68Å resolution

Crystal structure of the Q5LES9_BACFN protein from Bacteroides fragilis. Northeast Structural Genomics Consortium Target BfR250.

Released:
DOI: 10.2210/pdb3kkz/pdb
PDB entry 3kkz coloured by chain, front view.
PDB entry 3kkz coloured by chain, side view.
PDB entry 3kkz coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry authors: Vorobiev S, Neely H, Seetharaman J, Wang D, Ciccosanti C, Foote EL, Sahdev S, Acton TB, Xiao R, Everett JK, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-177486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.91 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5LES9 (Residues: 1-259; Coverage: 100%)
Gene name: BF9343_1590
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAM 2 x SAM
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 136.741Å b: 58.068Å c: 70.158Å
α: 90° β: 98.27° γ: 90°
R-values:
R R work R free
0.144 0.142 0.186
Expression system: Escherichia coli BL21(DE3)

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