› 3kmo

X-ray diffraction
2.6Å resolution

Crystal Structure of the Human GST Pi C47S/Y108V Double Mutant in Complex with the Ethacrynic Acid-Glutathione Conjugate (Grown in the Absence of the Reducing Agent DTT)

Released:
DOI: 10.2210/pdb3kmo/pdb
PDB entry 3kmo coloured by chain, front view.
PDB entry 3kmo coloured by chain, side view.
PDB entry 3kmo coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Parker LJ

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase P Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09211 (Residues: 2-210; Coverage: 100%)
Gene names: FAEES3, GST3, GSTP1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
2 bound ligands:
Ligand CA 6 x CA
Ligand EAA 2 x EAA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 75.902Å b: 89.348Å c: 69.383Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.169 0.165 0.254
Expression system: Escherichia coli

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