› 3kmp

X-ray diffraction
2.7Å resolution

Crystal Structure of SMAD1-MH1/DNA complex

Released:
DOI: 10.2210/pdb3kmp/pdb
PDB entry 3kmp coloured by chain, front view.
PDB entry 3kmp coloured by chain, side view.
PDB entry 3kmp coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of Smad1 MH1/DNA complex reveals distinctive rearrangements of BMP and TGF-beta effectors.
BabuRajendran N, Palasingam P, Narasimhan K, Sun W, Prabhakar S, Jauch R, Kolatkar PR
Nucleic Acids Res 38 3477-88 (2010)
PMID: 20147459

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116492 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mothers against decapentaplegic homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 14.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P70340 (Residues: 9-132; Coverage: 27%)
Gene names: Madh1, Madr1, Smad1
Sequence domains: MH1 domain
Structure domains: SMAD MH1 domain
5'-D(P*AP*TP*CP*AP*GP*TP*CP*TP*AP*GP*AP*CP*AP*TP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(P*GP*TP*AP*TP*GP*TP*CP*TP*AP*GP*AP*CP*TP*GP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.62 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 73.938Å b: 77.49Å c: 83.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.25
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

TGFβ signalling in context.
Massagué J. (2012)
6 more

6 mentions without citation

Structural determinants of Smad function in TGF-β signaling.
Macias et al. (2015)
5 more