› 3kmt

X-ray diffraction
1.78Å resolution

Crystal structure of vSET/SAH/H3 ternary complex

Released:
DOI: 10.2210/pdb3kmt/pdb
PDB entry 3kmt coloured by chain, front view.
PDB entry 3kmt coloured by chain, side view.
PDB entry 3kmt coloured by chain, top view.
Source organisms:
Primary publication:
Dimerization of a viral SET protein endows its function.
Wei H, Zhou MM
Proc Natl Acad Sci U S A 107 18433-8 (2010)
PMID: 20937900

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128783 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SET domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 119 amino acids
Theoretical weight: 13.61 KDa
Source organism: Paramecium bursaria Chlorella virus 1
UniProt:
  • Canonical: O41094 (Residues: 1-119; Coverage: 100%)
Gene name: A612L
Sequence domains: SET domain
Structure domains: SET domain
Histone H3.1 Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 8 amino acids
Theoretical weight: 816 Da
Source organism: Homo sapiens
UniProt:
  • Canonical: P68431 (Residues: 26-33; Coverage: 6%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand SAH 3 x SAH
1 modified residue:
Ligand MLZ 3 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 155.085Å b: 38.722Å c: 73.208Å
α: 90° β: 107.8° γ: 90°
R-values:
R R work R free
0.21 0.196 0.224

Quick links

Citations

3 review citations

EZH2: biology, disease, and structure-based drug discovery.
Tan et al. (2014)
2 more

2 mentions without citation

Dimerization of a viral SET protein endows its function.
Wei et al. (2010)
1 more