› 3kn1

X-ray diffraction
2.9Å resolution

Crystal Structure of Golgi Phosphoprotein 3 N-term Truncation Variant

Released:
DOI: 10.2210/pdb3kn1/pdb
PDB entry 3kn1 coloured by chain, front view.
PDB entry 3kn1 coloured by chain, side view.
PDB entry 3kn1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
PtdIns4P recognition by Vps74/GOLPH3 links PtdIns 4-kinase signaling to retrograde Golgi trafficking.
Wood CS, Schmitz KR, Bessman NJ, Setty TG, Ferguson KM, Burd CG
J Cell Biol 187 967-75 (2009)
PMID: 20026658

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo 24-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-190606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Golgi phosphoprotein 3 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H4A6 (Residues: 52-298; Coverage: 83%)
Gene names: GOLPH3, GPP34
Sequence domains: Golgi phosphoprotein 3 (GPP34)
Structure domains: yeast vps74-n-term truncation variant domain like

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I432
Unit cell:
a: 174.369Å b: 174.369Å c: 174.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.266
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
37 more

6 mentions without citation

Conditional peripheral membrane proteins: facing up to limited specificity.
Moravcevic et al. (2012)
5 more