› 3ko2

X-ray diffraction
2.9Å resolution

I-MsoI re-designed for altered DNA cleavage specificity (-7C)

Released:
DOI: 10.2210/pdb3ko2/pdb
PDB entry 3ko2 coloured by chain, front view.
PDB entry 3ko2 coloured by chain, side view.
PDB entry 3ko2 coloured by chain, top view.
Source organism: Monomastix sp. OKE-1
Primary publication:
Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.
Ashworth J, Taylor GK, Havranek JJ, Quadri SA, Stoddard BL, Baker D
Nucleic Acids Res 38 5601-8 (2010)
PMID: 20435674

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110996 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chains: A, B, F, G
Molecule details ›
Chains: A, B, F, G
Length: 170 amino acids
Theoretical weight: 19.6 KDa
Source organism: Monomastix sp. OKE-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0JWR6 (Residues: 1-170; Coverage: 100%)
Gene name: orf170
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*GP*CP*AP*GP*AP*CP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3' Chains: C, H
Molecule details ›
Chains: C, H
Length: 24 nucleotides
Theoretical weight: 7.4 KDa
Source organism: Monomastix sp. OKE-1
Expression system: Not provided
5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*GP*TP*CP*TP*GP*C)-3' Chains: D, I
Molecule details ›
Chains: D, I
Length: 24 nucleotides
Theoretical weight: 7.35 KDa
Source organism: Monomastix sp. OKE-1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 49.031Å b: 70.006Å c: 72.258Å
α: 81.66° β: 70° γ: 89.48°
R-values:
R R work R free
0.252 0.248 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

Macromolecular modeling and design in Rosetta: recent methods and frameworks.
Leman et al. (2020)
21 more

1 mention without citation

Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.
Ashworth et al. (2010)