› 3kqf

X-ray diffraction
1.8Å resolution

1.8 Angstrom Resolution Crystal Structure of Enoyl-CoA Hydratase from Bacillus anthracis.

Released:
DOI: 10.2210/pdb3kqf/pdb
PDB entry 3kqf coloured by chain, front view.
PDB entry 3kqf coloured by chain, side view.
PDB entry 3kqf coloured by chain, top view.
Source organism: Bacillus anthracis
Entry authors: Minasov G, Halavaty A, Wawrzak Z, Skarina T, Onopriyenko O, Papazisi L, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase/isomerase family protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 265 amino acids
Theoretical weight: 29.1 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L8PDF4 (Residues: 1-262; Coverage: 100%)
Gene name: GBAA_2551
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 73.747Å b: 130.721Å c: 73.802Å
α: 90° β: 114.47° γ: 90°
R-values:
R R work R free
0.157 0.156 0.193
Expression system: Escherichia coli

Quick links