› 3ksk

X-ray diffraction
2.35Å resolution

Crystal Structure of single chain PvuII

Released:
DOI: 10.2210/pdb3ksk/pdb
PDB entry 3ksk coloured by chain, front view.
PDB entry 3ksk coloured by chain, side view.
PDB entry 3ksk coloured by chain, top view.
Source organism: Proteus hauseri
Entry authors: Meramveliotaki C, Hountas A, Eliopoulos E, Kokkinidis M

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-150035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme PvuII Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.69 KDa
Source organism: Proteus hauseri
Expression system: Escherichia coli
UniProt:
  • Canonical: P23657 (Residues: 2-157; Coverage: 99%)
Gene name: pvuIIR
Sequence domains: Restriction endonuclease PvuII

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand MPD 1 x MPD
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P42
Unit cell:
a: 101.921Å b: 101.921Å c: 100.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.215 0.283
Expression system: Escherichia coli

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