› 3ktc

X-ray diffraction
1.54Å resolution

Crystal structure of Putative sugar isomerase (YP_050048.1) from ERWINIA CAROTOVORA ATROSEPTICA SCRI1043 at 1.54 A resolution

Released:
DOI: 10.2210/pdb3ktc/pdb
PDB entry 3ktc coloured by chain, front view.
PDB entry 3ktc coloured by chain, side view.
PDB entry 3ktc coloured by chain, top view.
Source organism: Pectobacterium atrosepticum SCRI1043
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
D-apiose = apulose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-apiose isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 37.5 KDa
Source organism: Pectobacterium atrosepticum SCRI1043
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6D5T7 (Residues: 1-332; Coverage: 100%)
Gene names: ECA1953, apsI
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

5 bound ligands:
Ligand FE 4 x FE
Ligand MG 2 x MG
Ligand CL 2 x CL
Ligand UNL 3 x UNL
Ligand EDO 15 x EDO
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3121
Unit cell:
a: 79.653Å b: 79.653Å c: 194.406Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.137 0.136 0.157
Expression system: Escherichia coli

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