› 3ktn

X-ray diffraction
2.26Å resolution

Crystal Structure of a putative 2-Keto-3-deoxygluconate Kinase from Enterococcus faecalis

Released:
DOI: 10.2210/pdb3ktn/pdb
PDB entry 3ktn coloured by chain, front view.
PDB entry 3ktn coloured by chain, side view.
PDB entry 3ktn coloured by chain, top view.
Source organism: Enterococcus faecalis
Entry authors: Satyanarayana L, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 39.68 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q837J7 (Residues: 2-336; Coverage: 100%)
Gene name: EF_0840
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6322
Unit cell:
a: 145.564Å b: 145.564Å c: 126.401Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.223 0.238
Expression system: Escherichia coli BL21(DE3)

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