› 3ku2

X-ray diffraction
2.3Å resolution

Crystal Structure of inactivated form of CDPK1 from toxoplasma gondii, TGME49.101440

Released:
DOI: 10.2210/pdb3ku2/pdb
PDB entry 3ku2 coloured by chain, front view.
PDB entry 3ku2 coloured by chain, side view.
PDB entry 3ku2 coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.
Wernimont AK, Artz JD, Finerty P, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R
Nat Struct Mol Biol 17 596-601 (2010)
PMID: 20436473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-domain protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 507 amino acids
Theoretical weight: 57.33 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BJF5 (Residues: 1-507; Coverage: 100%)
Gene name: CDPK1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand UNX 3 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 48.01Å b: 72.912Å c: 65.018Å
α: 90° β: 96.26° γ: 90°
R-values:
R R work R free
0.198 0.198 0.236
Expression system: Escherichia coli

Quick links

Citations

34 review citations

CDPKs in immune and stress signaling.
Boudsocq et al. (2013)
33 more

9 mentions without citation

Designing selective inhibitors for calcium-dependent protein kinases in apicomplexans.
Hui et al. (2015)
8 more