› 3kwc

X-ray diffraction
2Å resolution

Oxidized, active structure of the beta-carboxysomal gamma-Carbonic Anhydrase, CcmM

Released:
DOI: 10.2210/pdb3kwc/pdb
PDB entry 3kwc coloured by chain, front view.
PDB entry 3kwc coloured by chain, side view.
PDB entry 3kwc coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
Structural basis of the oxidative activation of the carboxysomal gamma-carbonic anhydrase, CcmM.
Peña KL, Castel SE, de Araujo C, Espie GS, Kimber MS
Proc Natl Acad Sci U S A 107 2455-60 (2010)
PMID: 20133749

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxysome assembly protein CcmM Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 229 amino acids
Theoretical weight: 24.82 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DKB5 (Residues: 1-209; Coverage: 32%)
Gene names: ccmM, tll0944
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

3 bound ligands:
Ligand IPA 7 x IPA
Ligand ZN 6 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 63.9Å b: 105.04Å c: 196.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.247
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Frontiers, opportunities, and challenges in biochemical and chemical catalysis of CO2 fixation.
Appel et al. (2013)
30 more

8 mentions without citation

Functions, compositions, and evolution of the two types of carboxysomes: polyhedral microcompartments that facilitate CO2 fixation in cyanobacteria and some proteobacteria.
Rae et al. (2013)
7 more