› 3kxp

X-ray diffraction
2.26Å resolution

Crystal Structure of E-2-(Acetamidomethylene)succinate Hydrolase

Released:
DOI: 10.2210/pdb3kxp/pdb
PDB entry 3kxp coloured by chain, front view.
PDB entry 3kxp coloured by chain, side view.
PDB entry 3kxp coloured by chain, top view.
Source organism: Mesorhizobium loti
Primary publication:
Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase.
McCulloch KM, Mukherjee T, Begley TP, Ealick SE
Biochemistry 49 1226-35 (2010)
PMID: 20099871

Function and Biology Details

Reaction catalysed: 
2-(acetamidomethylene)succinate + 2 H(2)O = acetate + succinate semialdehyde + NH(3) + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-(acetamidomethylene)succinate hydrolase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 314 amino acids
Theoretical weight: 34.15 KDa
Source organism: Mesorhizobium loti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q988D4 (Residues: 1-278; Coverage: 100%)
Gene name: mlr6787
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand CL 12 x CL
1 modified residue:
Ligand MSE 72 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C, APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 115.2Å b: 178.53Å c: 189.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.245
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The unusual extended C-terminal helix of the peroxisomal α/β-hydrolase Lpx1 is involved in dimer contacts but dispensable for dimerization.
Thoms et al. (2011)
2 more

4 mentions without citation

Crystal structure of a novel esterase Rv0045c from Mycobacterium tuberculosis.
Zheng et al. (2011)
3 more