3kye

X-ray diffraction
2.15Å resolution

Crystal Structure of Roadblock/LC7 Domain from Streptomyces avermitilis

Released:
Entry authors: Kim Y, Xu X, Cui H, Ng J, Edwards A, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-182673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Roadblock/LAMTOR2 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 150 amino acids
Theoretical weight: 16.18 KDa
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82MM8 (Residues: 9-134; Coverage: 88%)
Gene names: SAVERM_1632, cvnB3
Sequence domains: Roadblock/LC7 domain
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 66.422Å b: 155.341Å c: 48.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.227
Expression system: Escherichia coli