› 3kz9

X-ray diffraction
2.1Å resolution

Crystal structure of the master transcriptional regulator, SmcR, in Vibrio vulnificus provides insight into its DNA recognition mechanism

Released:
DOI: 10.2210/pdb3kz9/pdb
PDB entry 3kz9 coloured by chain, front view.
PDB entry 3kz9 coloured by chain, side view.
PDB entry 3kz9 coloured by chain, top view.
Source organism: Vibrio vulnificus
Primary publication:
Crystal structure of SmcR, a quorum-sensing master regulator of Vibrio vulnificus, provides insight into its regulation of transcription.
Kim Y, Kim BS, Park YJ, Choi WC, Hwang J, Kang BS, Oh TK, Choi SH, Kim MH
J Biol Chem 285 14020-30 (2010)
PMID: 20178981

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 206 amino acids
Theoretical weight: 24.05 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L8G8 (Residues: 1-205; Coverage: 100%)
Gene names: CRN52_17705, I7730_19665, XM75_c12331, smcR, vvpR
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P212121
Unit cell:
a: 78.494Å b: 99.025Å c: 129.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.198 0.251
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The TetR family of regulators.
Cuthbertson et al. (2013)
2 more

8 mentions without citation

Quorum Sensing Gene Regulation by LuxR/HapR Master Regulators in Vibrios.
Ball et al. (2017)
7 more